The Circular Dichroism Spectroscopy Technique to Study DNA-Protein Interactions
نویسندگان
چکیده
منابع مشابه
In vitro study of drug-protein interaction using electronic absorption, fluorescence, and circular dichroism spectroscopy
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Binding of RecA to poly(dG-m5dC) and poly(dG-dC) under B- and Z-form conditions was studied using circular dichroism (CD) and linear dichroism (LD). LD revealed a quantitative binding of RecA to Mg2+-induced Z-form poly(dG-m5dC) with a stoichiometry of 3.1 base pairs/RecA monomer, which is slightly larger than the 2.7 base pairs observed for the B-form. The LD spectra indicate a preferentially ...
متن کاملin vitro study of drug-protein interaction using electronic absorption, fluorescence, and circular dichroism spectroscopy
in the near future, design of a new generation of drugs targeting proteins will be required. considering the complex bond between the drug and protein, the structure and stability of the target protein should be considered. so far, a series of in vitro investigations have been conducted with the aim of predicting drug-biological medium interactions. in these studies, use of spectroscopic method...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2023
ISSN: ['1940-087X']
DOI: https://doi.org/10.3791/21469